Library Construction Strain Box 1

All in MC4100, pSB1A2 unless otherwise specified.

1	........									........
11		CW25553	pJAT8	BL21(DE3)	pLysS	I13401	I13401 (4A2)	I13401 (2K3)	I13401 (3K3)
21		I13402 (2K3)	I13403 (1AK3)	I13404	I13405		I13407	I13408
31		I13410 (1AK3)		I13412	I13413
51		I0500 (2K3)	I6300 (DH5a)	I6301	Q04400 (2K3)	I15015	I15016	I15017
61		I13404 (DH5a)	I13405 (DH5a)	I13450 (1A3)	I13452 (1A3)
71		I13500		I13502	I13503	I13504	I13505
81
91

	Number of Transformations
Reagents	1	5 (6X)	10 (11X)
PEG 3500 50% w/v	240 µl	1440 µl	2640 µl
LiAc 1.0 M	36 µl	216 µl	396 µl
Boiled SS-carrier DNA	50 µl	300 µl	550 µl
Plasmid DNA plus Water	34 µl	204 µl	374 µl
Total	360 µl	2160 µl	3960 µl

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