Lidstrom:Assaying enzyme libraries in 96-well plates: Difference between revisions
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== Protein Purification == | |||
* Pierce/Thermo Scientific [http://www.piercenet.com/files/1602015_PurifHB_INT.pdf Protein Purification Handbook] | |||
== Equipment/Supplies == | == Equipment/Supplies == | ||
=== General Supply Info === | |||
* Plates: | * Plates: | ||
** [http://www.tslabor.hu/depo/PCR-2012/Thermo%20Scientific%20Plate%20Guide.pdf Thermo Scientific Plate Guide] | ** [http://www.tslabor.hu/depo/PCR-2012/Thermo%20Scientific%20Plate%20Guide.pdf Thermo Scientific Plate Guide] to teach you about all the options for plates. Doesn't include half-area well plates. | ||
* Plate seals: | * Plate seals: | ||
** There are many kinds. A sample of all the types and their applications: [http://www.excelscientific.com/Excel_Decision_Tree_130209.pdf Excel brand flow chart] | ** There are many kinds. A sample of all the types and their applications: [http://www.excelscientific.com/Excel_Decision_Tree_130209.pdf Excel brand flow chart] | ||
=== Particularly inexpensive supplies === | |||
* Grainger for 96-well plates | |||
* Amazon now carries a lot of lab supplies | |||
== Growth in 96-Well Plates== | |||
=== Titramax-101 === | |||
*Maximum shaking speed as a function of volume: | |||
**[[image:96-wellplot.png|thumb|upright=3.0|center|When judging maximum shaking speed, we are aiming to avoid any intense rattling of the shaker on the lab bench. ]] | |||
=== Flask shaker w/ plate attachment === | |||
* maximum well volume as a function of shaking RPM: | |||
Latest revision as of 20:57, 12 November 2014
Protein Purification
- Pierce/Thermo Scientific Protein Purification Handbook
Equipment/Supplies
General Supply Info
- Plates:
- Thermo Scientific Plate Guide to teach you about all the options for plates. Doesn't include half-area well plates.
- Plate seals:
- There are many kinds. A sample of all the types and their applications: Excel brand flow chart
Particularly inexpensive supplies
- Grainger for 96-well plates
- Amazon now carries a lot of lab supplies
Growth in 96-Well Plates
Titramax-101
- Maximum shaking speed as a function of volume:
When judging maximum shaking speed, we are aiming to avoid any intense rattling of the shaker on the lab bench.
Flask shaker w/ plate attachment
- maximum well volume as a function of shaking RPM:
