Lidstrom:Autoclave

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Back to [[Lidstrom:Protocols|Protocols]]
Back to [[Lidstrom:Protocols|Protocols]]
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== Large Autoclave (room 435: by media prep room) ==
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(currently incomplete)
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*Ask Lab Manager for instruction before using for first time!
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*Do not open door during a run. Escaping Steam presents a burn risk!
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*If there is an emergency, turn autoclave off and leave it to vent itself!
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*Always use log book
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Small autoclave by RNA room:
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#Turn autoclave on
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#Select cycle
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##Cycle 1: Media less than 1L
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##Cycle 2: Media 1L or more
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##Cycle 3: Glassware
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##Cycle 4: Biological waste
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#Put items into autoclave in Nalgene autoclavable trays
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#Close door
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#Wait for ready light to come on
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#Press start button
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#Do not open door until cycle is fully complete
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#Open door a crack to and let stem vent
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#Turn off autoclave when finsihed
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1. Make sure the internal water level is up to the platform the
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== Small Autoclave (Room 409 by RNA room) ==
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autoclave racks sit on.
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*Ask Lab Manager for instruction before using for first time!
 +
*Do not open door during a run. Escaping Steam presents a burn risk!
 +
*If there is an emergency, turn autoclave off and leave it to vent itself!
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2. Make sure the external water reservoir is between the low and the high mark.
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#Make sure that the internal water level inside the autoclave chamber is even with top of metal platform.
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##If it is not fill with dH2O until it is.  This will ensure your samples get autoclaved, and not burnt.
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This will ensure your samples get autoclaved, and not burnt.
+
#Make sure external reservoir is filled so that the water level is between the high and low mark
 +
##If it is not fill with dH2O or empty so that it is
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#Place samples to be autoclaved into the chamber using metal cages to stack multiple samples
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#Shut door and turn wheel until door is firmly locked
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#Place magnetic key into receptacle
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#Turn autoclave on
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#Adjust time and temperature
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#Press the start button
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#Wait till run is completely finished before opening door
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##Do not open door if red light is flashing, or the pressure gauge is above zero
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#Turn off autoclave when finished

Revision as of 15:47, 5 July 2012

Back to Protocols

Large Autoclave (room 435: by media prep room)

  • Ask Lab Manager for instruction before using for first time!
  • Do not open door during a run. Escaping Steam presents a burn risk!
  • If there is an emergency, turn autoclave off and leave it to vent itself!
  • Always use log book
  1. Turn autoclave on
  2. Select cycle
    1. Cycle 1: Media less than 1L
    2. Cycle 2: Media 1L or more
    3. Cycle 3: Glassware
    4. Cycle 4: Biological waste
  3. Put items into autoclave in Nalgene autoclavable trays
  4. Close door
  5. Wait for ready light to come on
  6. Press start button
  7. Do not open door until cycle is fully complete
  8. Open door a crack to and let stem vent
  9. Turn off autoclave when finsihed

Small Autoclave (Room 409 by RNA room)

  • Ask Lab Manager for instruction before using for first time!
  • Do not open door during a run. Escaping Steam presents a burn risk!
  • If there is an emergency, turn autoclave off and leave it to vent itself!
  1. Make sure that the internal water level inside the autoclave chamber is even with top of metal platform.
    1. If it is not fill with dH2O until it is. This will ensure your samples get autoclaved, and not burnt.
  2. Make sure external reservoir is filled so that the water level is between the high and low mark
    1. If it is not fill with dH2O or empty so that it is
  3. Place samples to be autoclaved into the chamber using metal cages to stack multiple samples
  4. Shut door and turn wheel until door is firmly locked
  5. Place magnetic key into receptacle
  6. Turn autoclave on
  7. Adjust time and temperature
  8. Press the start button
  9. Wait till run is completely finished before opening door
    1. Do not open door if red light is flashing, or the pressure gauge is above zero
  10. Turn off autoclave when finished
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