Lidstrom:Digestion with Restriction Enzymes

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== Recipe ==
== Recipe ==
*Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends:
*Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends:
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**10 uL DNA  (totaling 500+ ng is good)
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**10+ uL DNA  (
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*** Want at least 500 ng.  Can elute a miniprep in 38 uL of water and use that whole thing.  Don't use miniprep DNA eluted in buffer; only use DNA eluted in water. 
**5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
**5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
**0.5 uL BSA (if required. See above)
**0.5 uL BSA (if required. See above)

Revision as of 18:04, 2 October 2012

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Recipe

  • If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.

Temperature/Time

  • 1 - 2 hours at 37oC or room temperature overnight
  • Inactivate by ..
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