Lidstrom:Digestion with Restriction Enzymes

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• Try it quick and imprecise like iGEM 2011 UW recommends:

10 uL DNA 5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers) 0.5 uL BSA (if required. See above) 1uL enzyme 1 water to 50 uL (32.5 uL, add first) If two enzymes are required, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp for buffer, temperature, BSA specifications.