Lidstrom:Diluting Primers

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Back to: Lidstrom Protocols

Primer Dilutions: People generally dilute dehydrated primers that arrive in the mail to 100uM, and use a working concentration of 10 uM.

To get 100uM: dissolve dehydrated primer with in 10 uL per nmole: For eg., for 25 nmoles of primer add 250 ul of water This will give you a concentration of 0.0001 M or 0.1 mM (1 nmole/10uL) = (1 nmole/10uL)*(10^6 uL/L)*(10^-9 mole/nmole) = 0.0001M = 0.1 mM = 100 uM

To prepare primer for use: Dilute this stock 1:10, to give a concentration of 10 uM

From this, use __ ml in a typical __ uL PCR reaction. This will give you a final concentration of __ pmoles in a PCR reaction.

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