Lidstrom:E. coli Vector Compatibility: Difference between revisions
| Line 1: | Line 1: | ||
Back to [[Lidstrom:Protocols|Protocols]] | Back to [[Lidstrom:Protocols|Protocols]] | ||
== | == Background == | ||
From Strategies for protein coexpression in Escherichia coli, 2006: | From Strategies for protein coexpression in Escherichia coli, 2006: | ||
Maintenance of multiple plasmids in E. coli is dependent on the plasmids possessing compatible origins of replication6 and different antibiotic resistance genes for selection (Table 1). If two plasmids containing the same origin of replication are used to cotransform E. coli, the replication of one plasmid will be inhibited as a result of an RNA antisense mechanism, which causes plasmid segregation within the population6,7. Compatible plasmids stably coexist in a single cell by occupying different subcellular locations within the bacterium8. Plasmid segregation can also occur if the plasmids carry the same antibiotic resistance gene; only one plasmid needs to be maintained to confer resistance. | Maintenance of multiple plasmids in E. coli is dependent on the plasmids possessing compatible origins of replication6 and different antibiotic resistance genes for selection (Table 1). If two plasmids containing the same origin of replication are used to cotransform E. coli, the replication of one plasmid will be inhibited as a result of an RNA antisense mechanism, which causes plasmid segregation within the population6,7. Compatible plasmids stably coexist in a single cell by occupying different subcellular locations within the bacterium8. Plasmid segregation can also occur if the plasmids carry the same antibiotic resistance gene; only one plasmid needs to be maintained to confer resistance. | ||
Revision as of 08:29, 14 October 2014
Back to Protocols
Background
From Strategies for protein coexpression in Escherichia coli, 2006: Maintenance of multiple plasmids in E. coli is dependent on the plasmids possessing compatible origins of replication6 and different antibiotic resistance genes for selection (Table 1). If two plasmids containing the same origin of replication are used to cotransform E. coli, the replication of one plasmid will be inhibited as a result of an RNA antisense mechanism, which causes plasmid segregation within the population6,7. Compatible plasmids stably coexist in a single cell by occupying different subcellular locations within the bacterium8. Plasmid segregation can also occur if the plasmids carry the same antibiotic resistance gene; only one plasmid needs to be maintained to confer resistance.
Common origins:
| copy # | present in these vectors | compatible with | comments | BioBrick origin equivalent | |
| pBR322 | ~ 20 per cell | pTrcHis2(A,B,C) | ColE1 == pBR322 | 1 | |
|---|---|---|---|---|---|
| CloDF13 | ~20–40 | pCDF-1b, pCDF duet, | ColE 1, P15A, RSF1030 |
More info from other sources
- From Addgene's Plasmids 101 free e-booklet:
pBR322
