Lidstrom:Gibson Assembly
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(New page: Design: - overlap by 20 bp Misc: - rxn product is salty: can be a problem for electrocompetent cells. Rob Egbert dilutes the competent cells when transforming with electroporation. Not ...) |
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Revision as of 10:39, 24 February 2012
Design: - overlap by 40 bp
Misc: - rxn product is salty: can be a problem for electrocompetent cells. Rob Egbert dilutes the competent cells when transforming with electroporation. Not so much a problem when using chemically competent cells. - Electrocompetence is ~ 1000x more effective so is tempting. -
