Lidstrom:PCR

Back to Protocols, Lidstrom

Taq

• Cheap
• Use for colony PCR (you don't keep the DNA so higher error rattes are ok)

Phusion

• faster, more accurate, & higher processiviy
• use for DNA modifications (e.g. things that will be put in vivo)
• more details & instructions: Phusion
• more stable (DNA/polymerase or tRNA/DNA?) interactions at high annealing temps (said Justin Siegal)
• Amanda heard that the Finnzymes version is better than NEB's. I couldn't find supporting information. (Janet)