Lidstrom:PCR
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Taq
- Cheap
- Use for colony PCR (you don't keep the DNA so higher error rattes are ok)
Phusion
- faster, more accurate, & higher processiviy
- use for DNA modifications (e.g. things that will be put in vivo)
- more details & instructions: Phusion
- more stable (DNA/polymerase or tRNA/DNA?) interactions at high annealing temps (said Justin Siegal)
- Amanda heard that the Finnzymes version is better than NEB's. I couldn't find supporting information. (Janet)