Lidstrom:Pouring Media Plates: Difference between revisions
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Critical: | Critical: | ||
*Put date on plates. Some antibiotics aren't very long lasting. | *Put date on plates. Some antibiotics aren't very long lasting. | ||
**For example, Ampicillin plates only last 2-3 weeks. | **For example, Ampicillin plates only last 2-3 weeks. (is there a citation for this? -Janet 2012/07/03) | ||
*Wait to put the antibiotic in until it is cool enough to touch the bottle to your cheek. | *Wait to put the antibiotic in until it is cool enough to touch the bottle to your cheek. | ||
**This prevents thermal degradation. | **This prevents thermal degradation. | ||
* If you tend to get bubbles appearing in the agar plates after incubating at 37oC: | |||
** Let the media cool more before pouring plates | |||
** "plates that have been stored in the refrigerator should be separated on a bench top and allowed to warm gradually to room temperature for several hours before incubating at 37 °C." -- [http://www.ncbi.nlm.nih.gov/pubmed/24203322 Sturdier 2014] | |||
Drew Endy has all the basics [[Endy:Pouring_LB_plates_from_prepped_media|here]] | Drew Endy has all the basics [[Endy:Pouring_LB_plates_from_prepped_media|here]] | ||
Latest revision as of 07:52, 31 March 2015
Back to Protocols
Critical:
- Put date on plates. Some antibiotics aren't very long lasting.
- For example, Ampicillin plates only last 2-3 weeks. (is there a citation for this? -Janet 2012/07/03)
- Wait to put the antibiotic in until it is cool enough to touch the bottle to your cheek.
- This prevents thermal degradation.
- If you tend to get bubbles appearing in the agar plates after incubating at 37oC:
- Let the media cool more before pouring plates
- "plates that have been stored in the refrigerator should be separated on a bench top and allowed to warm gradually to room temperature for several hours before incubating at 37 °C." -- Sturdier 2014
Drew Endy has all the basics here
