Lidstrom:Preparing Freezer Cell Stocks
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(New page: Back to protocols Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. Centrifuge for 5-10 minutes & pour off supernatant. Resuspend in 900 uL of HY (hyp...) |
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Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. | Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. | ||
Revision as of 21:27, 10 February 2012
Back to protocols
Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. Centrifuge for 5-10 minutes & pour off supernatant. Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Store at -80oC. Test stock after frozen a while.
