Lidstrom:Preparing Freezer Cell Stocks
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(New page: Back to protocols Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. Centrifuge for 5-10 minutes & pour off supernatant. Resuspend in 900 uL of HY (hyp...) |
Current revision (18:08, 1 March 2012) (view source) (→DMSO) |
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| - | Back to [[Lidstrom: Protocols|protocols]] | + | Back to [[Lidstrom:Protocols|protocols]] |
| - | Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. | + | ==DMSO== |
| - | Centrifuge for | + | *Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. |
| - | Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. | + | *Centrifuge for ~2+ minutes & pour off supernatant. |
| - | Store at -80oC. | + | *Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this; I hear it buffers. |
| - | Test stock after frozen a while. | + | *Store at -80oC. |
| + | *Test stock after frozen a while. | ||
| + | |||
| + | ==Glycerol== | ||
| + | * | ||
Current revision
Back to protocols
DMSO
- Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
- Centrifuge for ~2+ minutes & pour off supernatant.
- Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this; I hear it buffers.
- Store at -80oC.
- Test stock after frozen a while.
