Lidstrom:Preparing Freezer Cell Stocks: Difference between revisions
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Back to [[Lidstrom:Protocols|protocols]] | Back to [[Lidstrom:Protocols|protocols]] | ||
Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. | ==DMSO== | ||
Centrifuge for | *Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics. | ||
Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. | *Centrifuge for ~2+ minutes & pour off supernatant. | ||
Store at -80oC. | *Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this; I hear it buffers. | ||
Test stock after frozen a while. | *Store at -80oC. | ||
*Test stock after frozen a while. | |||
==Glycerol== | |||
* | |||
Revision as of 15:08, 1 March 2012
Back to protocols
DMSO
- Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
- Centrifuge for ~2+ minutes & pour off supernatant.
- Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this; I hear it buffers.
- Store at -80oC.
- Test stock after frozen a while.
