Lidstrom:Preparing Freezer Cell Stocks

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DMSO

• Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
• Centrifuge for ~2+ minutes & pour off supernatant.
• Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO. Hypho is better than LB for this; I hear it buffers.
• Store at -80oC.
• Test stock after frozen a while.