Lidstrom:Protocols
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==Cell Culture:== | ==Cell Culture:== | ||
*[[Lidstrom:Tube Spec|Tube Spec]] | *[[Lidstrom:Tube Spec|Tube Spec]] | ||
| + | *[[Lidstrom: Bioscreen|Bioscreen Setup]] | ||
| + | === Methylotrophs === | ||
| + | *[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | ||
| + | ===E. coli === | ||
*[[Lidstrom:M9|M9 recipe]] | *[[Lidstrom:M9|M9 recipe]] | ||
*[[Lidstrom:TB|Terrific Broth recipe]] | *[[Lidstrom:TB|Terrific Broth recipe]] | ||
| - | *[[Lidstrom: | + | *[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] |
==DNA/RNA:== | ==DNA/RNA:== | ||
| Line 22: | Line 26: | ||
====Isolation ==== | ====Isolation ==== | ||
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | *[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | ||
| + | *[[Lidstrom:Genomic DNA extraction|Genomic DNA minikit extraction]] | ||
| + | *[[Lidstrom:Genomic DNA extraction|Genomic DNA phenol/chloroform extraction]] | ||
| + | |||
====Manipulation==== | ====Manipulation==== | ||
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]] | *[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]] | ||
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| - | |||
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*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | *[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | ||
*[[Qiagen_Buffers| Qiagen Buffer Recipes]] | *[[Qiagen_Buffers| Qiagen Buffer Recipes]] | ||
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*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | *[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | ||
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | *[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | ||
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| + | ==== Restriction Cloning ==== | ||
| + | *[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]] | ||
| + | *[[Lidstrom:Ligation |Ligation (in progress)]] | ||
| + | |||
| + | ==== Gibson Cloning ==== | ||
| + | *[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | ||
====Obervation==== | ====Obervation==== | ||
Current revision
Back home: Lidstrom
Contents |
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
Methylotrophs
E. coli
DNA/RNA:
DNA
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
- PCR (in progress)
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
Protein
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
