Lidstrom:Protocols: Difference between revisions
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===RNA=== | ===RNA=== | ||
==E. Coli== | |||
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | |||
==Equipment Use== | ==Equipment Use== | ||
*[[Lidstrom:Baker Lab LCMS|Baker Lab LCMS]] | *[[Lidstrom:Baker Lab LCMS|Baker Lab LCMS]] |
Revision as of 15:26, 10 April 2012
Back home: Lidstrom
General Lab:
- Finding Chemicals
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
DNA/RNA:
DNA
- Intro: Building with DNA (in progress)
- Colony PCR
- Digestion with Restriction Enzymes
- Diluting Primers
- Gibson Assembly (In progress)
- Ligation (in progress)
- Loading Dye Recipe
- Miniprep (in progress)
- Oligo Orders (in progress)
- Overlap Extension PCR (in progress)
- PCR (in progress)
- Purifying DNA (in progress)
- Sequencing with GeneWiz
- Site-Directed Mutagenesis (in progress)
- Transformation (in progress)