Lidstrom:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
|||
| Line 38: | Line 38: | ||
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | *[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | ||
*[[Lidstrom:Transformation| Transformation (in progress)]] | *[[Lidstrom:Transformation| Transformation (in progress)]] | ||
===Enzyme Assays=== | |||
==General== | |||
*[[Lidstrom: Plate Reader| Plate Reader Setup]] | |||
==in vitro pathway assays (CO<sub>2</sub> project)== | ==in vitro pathway assays (CO<sub>2</sub> project)== | ||
*[[Lidstrom:in vitro Pathway Assay: Cell Prep| Cell Prep (in progress)]] | *[[Lidstrom:in vitro Pathway Assay: Cell Prep| Cell Prep (in progress)]] | ||
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | *[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | ||
Revision as of 14:38, 3 July 2012
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
- Intro: Building with DNA (in progress)
- Colony PCR
- Digestion with Restriction Enzymes
- Diluting Primers
- Gibson Assembly (In progress)
- Ligation (in progress)
- Loading Dye Recipe
- Miniprep (in progress)
- Oligo Orders (in progress)
- Overlap Extension PCR (in progress)
- PCR (in progress)
- Purifying DNA (in progress)
- Sequencing with GeneWiz
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
