Lidstrom:Protocols
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*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | *[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | ||
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] | *[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] | ||
| + | *[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | ||
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| + | ==Protein== | ||
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | *[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | ||
Revision as of 14:28, 5 July 2012
Back home: Lidstrom
Contents |
General Lab:
- Health & Safety
- Finding Chemicals
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
- Intro: Building with DNA (in progress)
- Colony PCR
- Digestion with Restriction Enzymes
- Diluting Primers
- Gibson Assembly (In progress)
- Ligation (in progress)
- Loading Dye Recipe
- Miniprep (in progress)
- Oligo Orders (in progress)
- Overlap Extension PCR (in progress)
- PCR (in progress)
- Purifying DNA (in progress)
- Sequencing with GeneWiz
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- P1 Phage Transduction (in progress)
