Lidstrom:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| (34 intermediate revisions by 2 users not shown) | |||
| Line 4: | Line 4: | ||
*[[Lidstrom:Health & Safety|Health & Safety]] | *[[Lidstrom:Health & Safety|Health & Safety]] | ||
*[[Lidstrom:Finding Chemicals|Finding Chemicals]] | *[[Lidstrom:Finding Chemicals|Finding Chemicals]] | ||
*[[Lidstrom:Dishwasher |Dishwasher]] | |||
*[[Lidstrom:Autoclave |Autoclave]] | *[[Lidstrom:Autoclave |Autoclave]] | ||
*[[Lidstrom:Sterile Technique | Sterile technique]] | *[[Lidstrom:Sterile Technique | Sterile technique]] | ||
| Line 12: | Line 13: | ||
==Cell Culture:== | ==Cell Culture:== | ||
*[[Lidstrom:Tube Spec|Tube Spec]] | *[[Lidstrom:Tube Spec|Tube Spec]] | ||
*[[Lidstrom:M9|M9 recipe]] | |||
*[[Lidstrom:TB|Terrific Broth recipe]] | |||
*[[Lidstrom: Bioscreen|Bioscreen Setup]] | |||
==DNA/RNA:== | ==DNA/RNA:== | ||
===DNA=== | ===DNA=== | ||
====Isolation ==== | |||
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | |||
*[[Lidstrom:Genomic DNA extraction|Genomic DNA extraction]] | |||
====Manipulation==== | |||
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]] | *[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]] | ||
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]] | *[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]] | ||
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | *[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | ||
*[[Lidstrom:Ligation |Ligation (in progress)]] | *[[Lidstrom:Ligation |Ligation (in progress)]] | ||
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | *[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | ||
*[[ | *[[Qiagen_Buffers| Qiagen Buffer Recipes]] | ||
=====Fancy Manipulation===== | |||
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | *[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | ||
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] | |||
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | |||
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | |||
====Obervation==== | |||
*[[Lidstrom:Colony PCR| Colony PCR]] | |||
*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]] | |||
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | |||
====Misc.==== | |||
*[[Lidstrom:PCR| PCR (in progress)]] | |||
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]] | |||
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | |||
*[[Lidstrom:Miniprep |Miniprep (in progress)]] | |||
*[[Lidstrom:Oligo Orders | Oligo Orders]] | |||
*[[Lidstrom:Diluting Primers | Diluting Primers]] | |||
==Protein== | |||
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | |||
==E. Coli== | ==E. Coli== | ||
*[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility (In Progress)]] | |||
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | *[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | ||
*[[Lidstrom:Transformation| Transformation (in progress)]] | *[[Lidstrom:Chemical Transformation|Chemical Transformation]] | ||
*[[Lidstrom:Electroporation|Electroporation]] | |||
*[[Lidstrom:Competent Cells|Competent Cell Lines]] | |||
==Enzyme Assays== | |||
===General=== | |||
*[[Lidstrom: Plate Reader| Plate Reader Setup]] | |||
===in vitro pathway assays (CO<sub>2</sub> project)=== | |||
*[[Lidstrom:in vitro Pathway Assay: Cell Prep| Cell Prep (in progress)]] | |||
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]] | |||
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]] | |||
== | == Metabolomics == | ||
=== CO2 project === | |||
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]] | |||
** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]] | |||
Revision as of 16:45, 4 February 2013
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
Isolation
Manipulation
- Intro: Building with DNA (in progress)
- Digestion with Restriction Enzymes
- Gibson Assembly (In progress)
- Ligation (in progress)
- Overlap Extension PCR (in progress)
- Qiagen Buffer Recipes
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Obervation
Misc.
- PCR (in progress)
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
Protein
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
