Lidstrom:Protocols

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*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
==DNA/RNA:==
==DNA/RNA:==
===DNA===
===DNA===
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====Isolation ====
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*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
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*[[Lidstrom:Genomic DNA extraction|Genomic DNA minikit extraction]]
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*[[Lidstrom:Genomic DNA extraction|Genomic DNA phenol/chloroform extraction]]
====Manipulation====
====Manipulation====
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
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*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 
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*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
-
*[[Lidstrom:Ligation |Ligation (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
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*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
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 +
==== Restriction Cloning ====
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*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
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*[[Lidstrom:Ligation |Ligation (in progress)]]
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==== Gibson Cloning ====
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*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
====Obervation====
====Obervation====
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== Metabolomics ==
== Metabolomics ==
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=== CO2 project ===
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
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** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]

Revision as of 10:07, 31 March 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools