Lidstrom:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
(Cell Culture:)
(22 intermediate revisions not shown.)
Line 13: Line 13:
==Cell Culture:==
==Cell Culture:==
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
 +
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
=== Methylotrophs ===
 +
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
 +
===E. coli === 
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
-
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
+
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
==DNA/RNA:==
==DNA/RNA:==
===DNA===
===DNA===
 +
====Isolation ====
 +
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
 +
*[[Lidstrom:Genomic DNA extraction|Genomic DNA minikit extraction]]
 +
*[[Lidstrom:Genomic DNA extraction|Genomic DNA phenol/chloroform extraction]]
====Manipulation====
====Manipulation====
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
-
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 
-
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
-
*[[Lidstrom:Ligation |Ligation (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 +
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
=====Fancy Manipulation=====
=====Fancy Manipulation=====
Line 33: Line 39:
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 +
 +
==== Restriction Cloning ====
 +
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 +
*[[Lidstrom:Ligation |Ligation (in progress)]]
 +
 +
==== Gibson Cloning ====
 +
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
====Obervation====
====Obervation====
Line 44: Line 57:
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
-
*[[Lidstrom:Oligo Orders | Oligo Orders (in progress)]]
+
*[[Lidstrom:Oligo Orders | Oligo Orders]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
*[[Lidstrom:Diluting Primers | Diluting Primers]]
Line 51: Line 64:
==E. Coli==
==E. Coli==
 +
*[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility  (In Progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
-
*[[Lidstrom:Transformation| Transformation (in progress)]]
+
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
 +
*[[Lidstrom:Electroporation|Electroporation]]
 +
*[[Lidstrom:Competent Cells|Competent Cell Lines]]
==Enzyme Assays==
==Enzyme Assays==
Line 63: Line 79:
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
 +
 +
== Metabolomics ==
 +
=== CO2 project ===
 +
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
 +
** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]

Revision as of 18:24, 2 May 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools