Lidstrom:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Isolation)
(Cell Culture:)
(4 intermediate revisions not shown.)
Line 13: Line 13:
==Cell Culture:==
==Cell Culture:==
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
 +
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
=== Methylotrophs ===
 +
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
 +
===E. coli === 
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
-
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
+
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
==DNA/RNA:==
==DNA/RNA:==
Line 22: Line 26:
====Isolation ====
====Isolation ====
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
-
*[[Lidstrom:Genomic DNA extraction|Genomic DNA extraction]]
+
*[[Lidstrom:Genomic DNA extraction|Genomic DNA minikit extraction]]
 +
*[[Lidstrom:Genomic DNA extraction|Genomic DNA phenol/chloroform extraction]]
====Manipulation====
====Manipulation====
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
-
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 
-
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
-
*[[Lidstrom:Ligation |Ligation (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
Line 37: Line 39:
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 +
 +
==== Restriction Cloning ====
 +
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 +
*[[Lidstrom:Ligation |Ligation (in progress)]]
 +
 +
==== Gibson Cloning ====
 +
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
====Obervation====
====Obervation====

Revision as of 18:24, 2 May 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools