Lidstrom:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(DNA/RNA:)
(Cell Culture:)
(One intermediate revision not shown.)
Line 13: Line 13:
==Cell Culture:==
==Cell Culture:==
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
 +
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
=== Methylotrophs ===
 +
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
 +
===E. coli === 
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
-
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
Line 30: Line 33:
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
 +
 +
=====Fancy Manipulation=====
 +
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
 +
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 +
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 +
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
==== Restriction Cloning ====
==== Restriction Cloning ====
Line 37: Line 46:
==== Gibson Cloning ====
==== Gibson Cloning ====
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
-
 
-
=====Fancy Manipulation=====
 
-
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
 
-
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 
-
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 
-
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 
====Obervation====
====Obervation====

Revision as of 17:24, 2 May 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools