Lidstrom:Protocols: Difference between revisions
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==Cell Culture:== | ==Cell Culture:== | ||
*[[Lidstrom:Tube Spec|Tube Spec]] | *[[Lidstrom:Tube Spec|Tube Spec]] | ||
*[[Lidstrom: Bioscreen|Bioscreen Setup]] | |||
=== Methylotrophs === | |||
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | |||
===E. coli === | |||
*[[Lidstrom:M9|M9 recipe]] | *[[Lidstrom:M9|M9 recipe]] | ||
*[[Lidstrom:TB|Terrific Broth recipe]] | *[[Lidstrom:TB|Terrific Broth recipe]] | ||
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] | *[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] | ||
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===DNA=== | ===DNA=== | ||
== PCR == | |||
*[[Lidstrom:Colony PCR| Colony PCR]] | |||
*[[Lidstrom:PCR| PCR (in progress)]] | |||
*[[Lidstrom:Thermocyclers|Thermocyclers]] | |||
====Isolation ==== | ====Isolation ==== | ||
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | *[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]] | ||
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*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | *[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | ||
*[[Qiagen_Buffers| Qiagen Buffer Recipes]] | *[[Qiagen_Buffers| Qiagen Buffer Recipes]] | ||
=====Fancy Manipulation===== | |||
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | |||
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] | |||
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | |||
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | |||
==== Restriction Cloning ==== | ==== Restriction Cloning ==== | ||
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==== Gibson Cloning ==== | ==== Gibson Cloning ==== | ||
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | *[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | ||
====Obervation==== | ====Obervation==== | ||
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====Misc.==== | ====Misc.==== | ||
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]] | *[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]] | ||
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | *[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]] | ||
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==Protein== | ==Protein== | ||
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | *[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | ||
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration | |||
==Buffers== | |||
*[[Lidstrom:Buffers|Buffers]] | |||
==E. Coli== | ==E. Coli== | ||
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*[[Lidstrom:Electroporation|Electroporation]] | *[[Lidstrom:Electroporation|Electroporation]] | ||
*[[Lidstrom:Competent Cells|Competent Cell Lines]] | *[[Lidstrom:Competent Cells|Competent Cell Lines]] | ||
== Lysing & Cells & Clarifying Extract == | |||
*[[Lidstrom:French Press|French Press]] | |||
*[[Lidstrom:Sonicator|Sonication]] | |||
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]] | |||
==Enzyme Assays== | ==Enzyme Assays== | ||
Revision as of 08:04, 13 June 2013
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
Methylotrophs
E. coli
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
Protein
- SDS-PAGE Protein Gels
- BCA assay to determine protein concentration
Buffers
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Lysing & Cells & Clarifying Extract
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
