Lidstrom:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (Isolation)
(Cell Culture:)
(15 intermediate revisions not shown.)
Line 13: Line 13:
==Cell Culture:==
==Cell Culture:==
*[[Lidstrom:Tube Spec|Tube Spec]]
*[[Lidstrom:Tube Spec|Tube Spec]]
 +
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
 +
=== Methylotrophs ===
 +
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
 +
===E. coli === 
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:M9|M9 recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom:TB|Terrific Broth recipe]]
-
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
Line 21: Line 25:
===DNA===
===DNA===
 +
== PCR ==
 +
*[[Lidstrom:Colony PCR| Colony PCR]]
 +
*[[Lidstrom:PCR| PCR (in progress)]]
 +
*[[Lidstrom:Thermocyclers|Thermocyclers]]
 +
====Isolation ====
====Isolation ====
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
Line 28: Line 37:
====Manipulation====
====Manipulation====
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
-
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 
-
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
-
*[[Lidstrom:Ligation |Ligation (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
Line 39: Line 45:
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 +
 +
==== Restriction Cloning ====
 +
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 +
*[[Lidstrom:Ligation |Ligation (in progress)]]
 +
 +
==== Gibson Cloning ====
 +
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
====Obervation====
====Obervation====
Line 46: Line 59:
====Misc.====
====Misc.====
-
*[[Lidstrom:PCR| PCR (in progress)]]
 
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]]
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]]
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
Line 55: Line 67:
==Protein==
==Protein==
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
 +
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
 +
 +
==Buffers==
 +
*[[Lidstrom:Buffers|Buffers]]
==E. Coli==
==E. Coli==
Line 63: Line 79:
*[[Lidstrom:Competent Cells|Competent Cell Lines]]
*[[Lidstrom:Competent Cells|Competent Cell Lines]]
-
==Enzyme Assays==
+
== Lysing & Cells & Clarifying Extract ==
 +
*[[Lidstrom:French Press|French Press]]
 +
*[[Lidstrom:Sonicator|Sonication]]
 +
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
 +
 
 +
==Assays==
===General===
===General===
-
*[[Lidstrom: Plate Reader| Plate Reader Setup]]
+
*[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
 +
*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
 +
*[[Lidstrom: Flow Cytometer| Flow Cytometer]]
===in vitro pathway assays (CO<sub>2</sub> project)===
===in vitro pathway assays (CO<sub>2</sub> project)===

Revision as of 18:25, 20 September 2013

Back home: Lidstrom

Contents

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Buffers

E. Coli

Lysing & Cells & Clarifying Extract

Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project

Personal tools