Lidstrom:Protocols: Difference between revisions
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*[[Lidstrom:Tube Spec|Tube Spec]] | *[[Lidstrom:Tube Spec|Tube Spec]] | ||
*[[Lidstrom: Bioscreen|Bioscreen Setup]] | *[[Lidstrom: Bioscreen|Bioscreen Setup]] | ||
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]] | |||
=== Methylotrophs === | === Methylotrophs === | ||
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | *[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | ||
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===General=== | ===General=== | ||
*[[Lidstrom: Plate Reader| Plate Reader Setup]] | *[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]] | ||
*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]] | |||
*[[Lidstrom: Flow Cytometer| Flow Cytometer]] | *[[Lidstrom: Flow Cytometer| Flow Cytometer]] | ||
Revision as of 15:25, 20 September 2013
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
Methylotrophs
E. coli
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
Protein
- SDS-PAGE Protein Gels
- BCA assay to determine protein concentration
Buffers
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Lysing & Cells & Clarifying Extract
Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
