Lidstrom:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Misc) |
|||
| (11 intermediate revisions by the same user not shown) | |||
| Line 15: | Line 15: | ||
*[[Lidstrom:Tube Spec|Tube Spec]] | *[[Lidstrom:Tube Spec|Tube Spec]] | ||
*[[Lidstrom: Bioscreen|Bioscreen Setup]] | *[[Lidstrom: Bioscreen|Bioscreen Setup]] | ||
=== Methylotrophs === | === Methylotrophs === | ||
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | *[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]] | ||
| Line 22: | Line 22: | ||
*[[Lidstrom:TB|Terrific Broth recipe]] | *[[Lidstrom:TB|Terrific Broth recipe]] | ||
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] | *[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]] | ||
=== OD measurements === | |||
* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]] | |||
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]] | |||
==DNA/RNA:== | ==DNA/RNA:== | ||
| Line 43: | Line 47: | ||
=====Fancy Manipulation===== | =====Fancy Manipulation===== | ||
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | *[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | ||
** [[Lidstrom:QuikChange Site-Directed Mutagenesis|QuikChange Site-Directed Mutagenesis]] | |||
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] | *[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] | ||
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | *[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | ||
| Line 58: | Line 63: | ||
*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]] | *[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]] | ||
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | *[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | ||
====Mutagenesis==== | |||
*[[Lidstrom:EMS Mutagenesis|EMS Mutagenesis]] | |||
*[[Lidstrom:UV Mutagenesis|UV Mutagenesis]] | |||
====Misc.==== | ====Misc.==== | ||
| Line 68: | Line 77: | ||
==Protein== | ==Protein== | ||
=== Purification === | |||
*[[Lidstrom:His-tag Protein Purification|His-tag Protein Purification]] | |||
=== Analytics === | |||
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | *[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | ||
| Line 99: | Line 112: | ||
===General=== | ===General=== | ||
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]] | *[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]] | ||
*[[Lidstrom:Enzyme Assay Data Analysis|Enzyme Assay Data Analysis]] | |||
*[[Lidstrom:Solution Stock Info|Solution Stock Info]] | *[[Lidstrom:Solution Stock Info|Solution Stock Info]] | ||
*[[Lidstrom:Path Length in microwell plates|Path Length in 96-well plates]] | |||
*[[Lidstrom:Reducing Agents|Reducing Agents]] | |||
=== Equipment === | === Equipment === | ||
| Line 119: | Line 135: | ||
==== Misc ==== | ==== Misc ==== | ||
*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]] | *[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]] | ||
*[[Lidstrom:Assaying enzyme libraries|Assaying enzyme libraries]] | |||
== Metabolomics == | == Metabolomics == | ||
Revision as of 11:25, 30 June 2014
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
- Filtration
Cell Culture:
Methylotrophs
E. coli
OD measurements
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Mutagenesis
Misc.
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
- NEB cloning guide
Protein
Purification
Analytics
Protein Concentration Determination
- Choosing a protein concentration quantification method
- BCA assay to determine protein concentration
- NanoDrop for proteins
Chemicals
- Chemical Stability
- Also, see Solution Stock Info
Buffers
E. coli
E. coli transformation
Lysing & Cells & Clarifying Extract
Assays
General
- Enzyme Assay Basics
- Enzyme Assay Data Analysis
- Solution Stock Info
- Path Length in 96-well plates
- Reducing Agents
Equipment
- Plate readers:
- Cuvette spectrophotometer:
- Centrifuge:
- Sorvall centrifuge goes faster than the regular centrifuges that allow 50mL tubes
- Flow Cytometry:
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
