Lidstrom:Protocols: Difference between revisions
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===E. coli === | ===E. coli === | ||
*[[Lidstrom:E. coli media comparison|E. coli media comparison]] | |||
*[[Lidstrom:Autoinduction Media|Autoinduction Media]] | *[[Lidstrom:Autoinduction Media|Autoinduction Media]] | ||
*[[Lidstrom:M9|M9 recipe]] | *[[Lidstrom:M9|M9 recipe]] | ||
| Line 80: | Line 81: | ||
*[[Lidstrom:Diluting Primers | Diluting Primers]] | *[[Lidstrom:Diluting Primers | Diluting Primers]] | ||
*[https://www.neb.com/~/media/NebUs/Files/Brochures/Cloning_Guide_1113.pdf NEB cloning guide] | *[https://www.neb.com/~/media/NebUs/Files/Brochures/Cloning_Guide_1113.pdf NEB cloning guide] | ||
==Gene Knockouts== | |||
*[[Lidstrom:Gene_Knockouts|Gene Knockouts]] | |||
==Protein== | ==Protein== | ||
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*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]] | *[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]] | ||
*[[Lidstrom:Assaying enzyme libraries|Assaying enzyme libraries]] | *[[Lidstrom:Assaying enzyme libraries|Assaying enzyme libraries]] | ||
==== Specific Assays ==== | |||
*[[Lidstrom:Formate Assay via Formate Dehdyrogenase|Formate Assay via Formate Dehdyrogenase]] | |||
== Metabolomics == | == Metabolomics == | ||
Revision as of 16:50, 21 October 2015
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
- Filtration
Cell Culture:
Methylotrophs
E. coli
- E. coli media comparison
- Autoinduction Media
- M9 recipe
- Terrific Broth recipe
- EZ amino acid stock recipe
OD measurements
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Mutagenesis
Misc.
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
- NEB cloning guide
Gene Knockouts
Protein
Purification
Analytics
Protein Concentration Determination
- Choosing a protein concentration quantification method
- BCA assay to determine protein concentration
- NanoDrop for proteins
Chemicals
- Chemical Stability
- Also, see Solution Stock Info
Buffers
E. coli
- E. coli Vector Compatibility (In Progress)
- origins of replication
- Competent Cell Preparation (in progress)
E. coli transformation
Lysing & Cells & Clarifying Extract
Assays
General
- Enzyme Assay Basics
- Enzyme Assay Data Analysis
- Solution Stock Info
- Path Length in 96-well plates
- Reducing Agents
Equipment
- Plate readers:
- Cuvette spectrophotometer:
- Centrifuge:
- Sorvall centrifuge goes faster than the regular centrifuges that allow 50mL tubes
- Flow Cytometry:
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
Misc
Specific Assays
Metabolomics
- Hot water extraction for in vivo metabolite measurement
- Hot water extraction for in vitro metabolite measurement
