Lidstrom:Protocols

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General Lab:

• Health & Safety
• Finding Chemicals
• Autoclave
• Sterile technique
• E. Coli basics
• Preparing Freezer Cell Stocks
• Pouring Media Plates

Cell Culture:

• Tube Spec
• M9 recipe
• Terrific Broth recipe
• Bioscreen Setup

DNA/RNA:

DNA

• Intro: Building with DNA (in progress)
• Colony PCR
• Digestion with Restriction Enzymes
• Diluting Primers
• Gibson Assembly (In progress)
• Ligation (in progress)
• Loading Dye Recipe
• Miniprep (in progress)
• Oligo Orders (in progress)
• Overlap Extension PCR (in progress)
• PCR (in progress)
• Purifying DNA (in progress)
• Sequencing with GeneWiz
• Site-Directed Mutagenesis (in progress)
• λ-Red Mediated Gene Knockouts (in progress)
• SDS-PAGE Protein Gels

E. Coli

• Competent Cell Preparation (in progress)
• Transformation (in progress)

Enzyme Assays

General

• Plate Reader Setup

in vitro pathway assays (CO₂ project)

• Cell Prep (in progress)
• Crude Extract Prep (in progress)
• Sample Analysis via UPLC-MS/MS (in progress)