Lidstrom:Reducing Agents
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Why are reducing agents used?
What reducing agents are commonly used?
- DTT
- the most popular reducing agent for proteins (citation)
- b-ME (β-mercaptoethanol)
- TCEP
- more expensive, odorless
Misc
- Side by Side comparison, DTT VS TCEP
- TCEP is not very stable in phosphate buffers, especially at neutral pH.
- Effective pH range is 1.5 to 8.5.