Lidstrom:Site-Directed Mutagenesis

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(New page: Back to Protocols ==Key Concepts== *There are several ways to change bases. Qucick-Change is the fast and standard way. *Some protocols use ligase. *Some protocol...)
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==Key Concepts==
==Key Concepts==
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*There are several ways to change bases.  Qucick-Change is the fast and standard way.  
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*There are several ways to change bases.  Quick-Change is the fast and standard way.  
*Some protocols use ligase.
*Some protocols use ligase.
*Some protocols allow you to do more than one change simultaneously.
*Some protocols allow you to do more than one change simultaneously.

Revision as of 10:41, 29 February 2012

Back to Protocols

Key Concepts

  • There are several ways to change bases. Quick-Change is the fast and standard way.
  • Some protocols use ligase.
  • Some protocols allow you to do more than one change simultaneously.

Kunkel Mutagenesis

  • Justin Siegel at the Baker lab is our local expert. The UW iGem team uses this method.
  • Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes.
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