Lidstrom:Site-Directed Mutagenesis
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(New page: Back to Protocols ==Key Concepts== *There are several ways to change bases. Qucick-Change is the fast and standard way. *Some protocols use ligase. *Some protocol...) |
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==Key Concepts== | ==Key Concepts== | ||
| - | *There are several ways to change bases. | + | *There are several ways to change bases. QuickChange is the fast and standard way. |
*Some protocols use ligase. | *Some protocols use ligase. | ||
| - | *Some protocols allow you to do more than one change simultaneously. | + | *Some protocols allow you to do more than one change simultaneously. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2629768/pdf/1472-6750-8-91.pdf Here] is an excellent article Amanda found about doing multiple mutations with QuickChange |
==Kunkel Mutagenesis== | ==Kunkel Mutagenesis== | ||
*Justin Siegel at the Baker lab is our local expert. The [http://2010.igem.org/Team:Washington/Protocols/Kunkel UW iGem] team uses this method. | *Justin Siegel at the Baker lab is our local expert. The [http://2010.igem.org/Team:Washington/Protocols/Kunkel UW iGem] team uses this method. | ||
*Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes. | *Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes. | ||
Current revision
Back to Protocols
Key Concepts
- There are several ways to change bases. QuickChange is the fast and standard way.
- Some protocols use ligase.
- Some protocols allow you to do more than one change simultaneously. Here is an excellent article Amanda found about doing multiple mutations with QuickChange
Kunkel Mutagenesis
- Justin Siegel at the Baker lab is our local expert. The UW iGem team uses this method.
- Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes.
