Lidstrom:Site-Directed Mutagenesis

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(Key Concepts)
(Key Concepts)
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==Key Concepts==
==Key Concepts==
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*There are several ways to change bases.  Quick-Change is the fast and standard way.  
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*There are several ways to change bases.  QuickChange is the fast and standard way.  
*Some protocols use ligase.
*Some protocols use ligase.
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*Some protocols allow you to do more than one change simultaneously.
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*Some protocols allow you to do more than one change simultaneously. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2629768/pdf/1472-6750-8-91.pdf Here] is an excellent article Amanda found about doing multiple mutations with QuickChange
==Kunkel Mutagenesis==
==Kunkel Mutagenesis==
*Justin Siegel at the Baker lab is our local expert.  The [http://2010.igem.org/Team:Washington/Protocols/Kunkel UW iGem] team uses this method.
*Justin Siegel at the Baker lab is our local expert.  The [http://2010.igem.org/Team:Washington/Protocols/Kunkel UW iGem] team uses this method.
*Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes.
*Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes.

Revision as of 10:44, 29 February 2012

Back to Protocols

Key Concepts

  • There are several ways to change bases. QuickChange is the fast and standard way.
  • Some protocols use ligase.
  • Some protocols allow you to do more than one change simultaneously. Here is an excellent article Amanda found about doing multiple mutations with QuickChange

Kunkel Mutagenesis

  • Justin Siegel at the Baker lab is our local expert. The UW iGem team uses this method.
  • Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes.
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