Lidstrom:Site-Directed Mutagenesis: Difference between revisions
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(New page: Back to Protocols ==Key Concepts== *There are several ways to change bases. Qucick-Change is the fast and standard way. *Some protocols use ligase. *Some protocol...) |
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Revision as of 07:41, 29 February 2012
Back to Protocols
Key Concepts
- There are several ways to change bases. Qucick-Change is the fast and standard way.
- Some protocols use ligase.
- Some protocols allow you to do more than one change simultaneously.
Kunkel Mutagenesis
- Justin Siegel at the Baker lab is our local expert. The UW iGem team uses this method.
- Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes.
