Lidstrom:Site-Directed Mutagenesis

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Key Concepts

  • There are several ways to change bases. QuickChange is the fast and standard way.
  • Some protocols use ligase.
  • Some protocols allow you to do more than one change simultaneously. Here is an excellent article Amanda found about doing multiple mutations with QuickChange

Kunkel Mutagenesis

  • Justin Siegel at the Baker lab is our local expert. The UW iGem team uses this method.
  • Our lab (at least Amanda and Janet) have not tried it yet. Our understanding is that it is for more substantial changes than just single amino acid changes.
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