Lidstrom:Sorvall Centrifuge: Difference between revisions
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== | == Background == | ||
[[image:Lidstrom_Sorvoll.png|thumb|upright=1.0|center|Lidstrom Sorvoll centrifuge]] | [[image:Lidstrom_Sorvoll.png|thumb|upright=1.0|center|Lidstrom Sorvoll centrifuge]] | ||
We have a Sorvoll RC-5B centrifuge that is useful for spinning large volumes in 0.5L bottles or medium-sized (a few mL) volumes very quickly | |||
== Rotors == | == Rotors == | ||
[http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors] | [http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors] | ||
=== SS-34 === | |||
* Holds 8*50 mL, spins at '''[http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf 20,000 RPM/36,900 RCF]''' | |||
** BUT Katya (circa 2010) recommends using it at <= 18,000 RPM | |||
**Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] | **Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] | ||
***Can't be autoclaved. | ***Can't be autoclaved. | ||
**** Actually, Amanda might have autoclaved them before. | **** Actually, Amanda might have autoclaved them before. | ||
***Can't write on body; can only write on cap. Sandy says stickers don't stick. | ***Can't write on body; can only write on cap. Sandy says stickers don't stick. | ||
[[image:SS-34 rotor speeds and k factors.png|thumb|upright=2.0|center|SS-34 rotor speeds and k factors]] | |||
=== GS-3 === | |||
* [http://www.thermoscientific.fr/eThermo/CMA/PDFs/Various/File_582.pdf GS-3]. | * [http://www.thermoscientific.fr/eThermo/CMA/PDFs/Various/File_582.pdf GS-3]. | ||
** For bigger bottles. | ** BUT Katya (circa 2010) recommends using it at <= 7,500 | ||
** For bigger bottles. (0.5L) | |||
** Holds eppendorf (1.5mL) tubes | ** Holds eppendorf (1.5mL) tubes | ||
* Spins at '''[http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf 9,000 RPM/13,700 RCF]''' | |||
[[image:GS-3 rotor speeds and k factors.png|thumb|upright=2.0|center|GS-34 rotor speeds and k factors]] | |||
== Explanation of RCF == | |||
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | |||
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF. | * For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF. | ||
*There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed. | *There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed. | ||
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]] | [[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]] | ||
== Instructions == | == Instructions == | ||
Revision as of 17:57, 21 January 2015
Back to Protocols
Background
We have a Sorvoll RC-5B centrifuge that is useful for spinning large volumes in 0.5L bottles or medium-sized (a few mL) volumes very quickly
Rotors
SS-34
- Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- BUT Katya (circa 2010) recommends using it at <= 18,000 RPM
- Tubes:
SS-34 tubes - Can't be autoclaved.
- Actually, Amanda might have autoclaved them before.
- Can't write on body; can only write on cap. Sandy says stickers don't stick.
- Can't be autoclaved.
GS-3
- GS-3.
- BUT Katya (circa 2010) recommends using it at <= 7,500
- For bigger bottles. (0.5L)
- Holds eppendorf (1.5mL) tubes
- Spins at 9,000 RPM/13,700 RCF
Explanation of RCF
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
- There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
Stoke's equation 
centrifugation speeds for applications
