Lidstrom:Sorvall Centrifuge: Difference between revisions
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** Rotors we have: | ** Rotors we have: | ||
*** SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF | *** SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF | ||
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] Can't be autoclaved. Can't write on body; can only write on cap. Sandy says | ****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] | ||
*****Can't be autoclaved. | |||
****** Actually, Amanda might have autoclaved them before. | |||
*****Can't write on body; can only write on cap. Sandy says stickers don't stick. | |||
*** GS-3. | *** GS-3. | ||
*** ____. Holds eppendorf (1.5mL) tubes | *** ____. Holds eppendorf (1.5mL) tubes | ||
Revision as of 16:58, 11 June 2014
Back to Protocols
Picture
Rotors
- Sorvall rotors
- Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Tubes:
SS-34 tubes - Can't be autoclaved.
- Actually, Amanda might have autoclaved them before.
- Can't write on body; can only write on cap. Sandy says stickers don't stick.
- Can't be autoclaved.
- Tubes:
- GS-3.
- ____. Holds eppendorf (1.5mL) tubes
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- Rotors we have:
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
There are stickers with maximum speeds. These were set by Katya, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
Stoke's equation 
centrifugation speeds for applications
