Lidstrom:Sorvall Centrifuge: Difference between revisions
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[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | [[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | ||
== Instructions == | |||
Example protocol for AM1: | |||
* | |||
Janet's SIP3-4 centrifugation: | |||
* Chill to 4<sup>o</sup>C with SS-34 rotor inside | |||
* Fill correct tubes | |||
* Spin for ___ min at ___ RCF at ___<sup>o</sup>C | |||
== Centrifugation Math & Basics == | == Centrifugation Math & Basics == | ||
Revision as of 10:28, 24 October 2013
Back to Protocols
Picture
Rotors
- Sorvall rotors
- Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- GS-3.
- ____. Holds eppendorf (1.5mL) tubes
- Rotors we have:
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC
Centrifugation Math & Basics
- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
Stoke's equation 
centrifugation speeds for applications
