Lidstrom:Thermocyclers: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 42: | Line 42: | ||
* ability to recall past thermocycling programs used | * ability to recall past thermocycling programs used | ||
** occasionally useful for troubleshooting failed reactions | ** occasionally useful for troubleshooting failed reactions | ||
* Optional window that shows how long until a protocol is compete | |||
** Marina's BioRad system's screen goes black with big light up numbers indicating how long until it is complete. It can be seen from several benches away. (This machine is no longer here.) |
Revision as of 08:42, 13 June 2013
Back to Protocols
Machines available as of 6/2013
Thermocycler location | Appearance | Brand | Capabilities | Manual | Use restrictions |
Janet's bench | black | DNA Engine: DYAD | small block doesn't do gradient; large block does | n/a | none |
Amanda's window | white & new | Eppendorf: Mastercycler gradient | gradient, but see manual b/c it is weird | Mastercycler Gradient | none |
Frances' window | black & old | DNA engine | no gradient | n/a | none |
Nate's Bench | ## | ## | ## | ## | none? |
Alexey's Bench | ## | ## | 2 blocks (gradient?) | ## | none? |
Ceci's window | white, small | Eppendorf: ?? | ?? | ?? | none? |
What makes for a good thermocycler?
Functions
- gradient block(s)
- multiple blocks on one machine
- reduces the need to enter programs into many machines
- allow for saving of protocols in folders
- different researchers can have different folders, preventing the accumulation of a huge list of protocols, and others accidentally writing over other peoples' protocols
Interface
- intuitive menu
- NOT true of eppendorf systems!
- BioRad system is fantastic
- Show what happens to each well for a given gradient setting *while* the protocol is being edited
- Not true of white eppendorf Mastercycler gradient
Bonus Points
- ability to recall past thermocycling programs used
- occasionally useful for troubleshooting failed reactions
- Optional window that shows how long until a protocol is compete
- Marina's BioRad system's screen goes black with big light up numbers indicating how long until it is complete. It can be seen from several benches away. (This machine is no longer here.)