Lidstrom:UV Mutagenesis: Difference between revisions
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*[http://www.photobiology.info/UV-Muta.html UV Radiation and Spontaneous Mutagenesis]: summary by Kendric C. Smith, Emeritus Professor, Radiation Oncology (Radiation Biology), Stanford University School of Medicine | *[http://www.photobiology.info/UV-Muta.html UV Radiation and Spontaneous Mutagenesis]: summary by Kendric C. Smith, Emeritus Professor, Radiation Oncology (Radiation Biology), Stanford University School of Medicine | ||
** Discusses repair mechanisms a lot. | ** Discusses repair mechanisms a lot. | ||
**"G:C -> A:T transitions predominate after UV irradiation. In E. coli the (6-4)-photoproduct may be more important for mutagenesis, while the pyrimidine dimer may be more important in mammalian cells. In human cells, mutations occur at the C of a TC, CT, or CC pyrimidine dimer, but not at TT dimers, and also occur at the C of TC and CC (6-4)-adducts (reviewed by Brash, 1988)." | ** UV radiation produces a preponderance of alterations in the pyrimidines, and the large majority of these products involve the linking of two adjacent pyrimidines | ||
** | **"G:C -> A:T transitions predominate after UV irradiation. | ||
***In E. coli the (6-4)-photoproduct may be more important for mutagenesis, while the pyrimidine dimer may be more important in mammalian cells. In human cells, mutations occur at the C of a TC, CT, or CC pyrimidine dimer, but not at TT dimers, and also occur at the C of TC and CC (6-4)-adducts (reviewed by Brash, 1988)." | |||
** UV-radiation-induced mutations show 2-hit kinetics at high doses | |||
*** i.e., they are produced as a function of the square of the dose | |||
** While UV irradiation does not produce DNA double-strand breaks directly, they can be formed as a consequence of the inefficient repair of overlapping excision gaps, and of overlapping daughter-strand gaps | |||
** The formation of DNA-protein cross-links has been shown to be of significance in the killing of E. coli | |||
** In general, UV irradiation produces mutations along a gene in a non-random manner, i.e., mutations are observed at certain base pairs more frequently than at others |
Revision as of 07:47, 1 July 2014
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- UV Radiation and Spontaneous Mutagenesis: summary by Kendric C. Smith, Emeritus Professor, Radiation Oncology (Radiation Biology), Stanford University School of Medicine
- Discusses repair mechanisms a lot.
- UV radiation produces a preponderance of alterations in the pyrimidines, and the large majority of these products involve the linking of two adjacent pyrimidines
- "G:C -> A:T transitions predominate after UV irradiation.
- In E. coli the (6-4)-photoproduct may be more important for mutagenesis, while the pyrimidine dimer may be more important in mammalian cells. In human cells, mutations occur at the C of a TC, CT, or CC pyrimidine dimer, but not at TT dimers, and also occur at the C of TC and CC (6-4)-adducts (reviewed by Brash, 1988)."
- UV-radiation-induced mutations show 2-hit kinetics at high doses
- i.e., they are produced as a function of the square of the dose
- While UV irradiation does not produce DNA double-strand breaks directly, they can be formed as a consequence of the inefficient repair of overlapping excision gaps, and of overlapping daughter-strand gaps
- The formation of DNA-protein cross-links has been shown to be of significance in the killing of E. coli
- In general, UV irradiation produces mutations along a gene in a non-random manner, i.e., mutations are observed at certain base pairs more frequently than at others