Lidstrom: Agarose Gel Electrophoresis

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• handy link: gel electrophoresis
  • click the gel electrophoresis button on the left

Starting with an empty box

• Fill with TBE until it covers the gel holding tray.
• Add 40 uL of ethidium bromide if your gels don't already have it within
  • If your buffer already has some ethidium bromide but it needs more, 20 uL is probably good.
• Load sample
  • 1.7 uL of a PCR product is usually plenty
• Load ladder
  • If using MassRuler, 4 uL is good for skinny lanes.
• Run @ 110 V for 20 min and check the gel.
  • 40 min is pretty safe for 0.7 - 1% agarose gels