Liphardt: Antibiotics

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'''Ampicillin'''<br>
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<table border="1" cellpadding="5" cellspacing="0">
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50 ug/mL<br>
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Stock: dissolve salt (not free base) 50 mg/mL in millipore water, filter sterilize, store at -20°<br>
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Store plates/media at 4°, good for 2 months<br>
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'''Kanamycin'''<br>
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<tr>
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25 ug/mL<br>
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<td>Antibiotic</td>
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Store plates/media at 4°, good for 1 month<br>
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<td>Typical Concentration</td>
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<td>Store At</td>
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<td>Good For</td>
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<td>Molecular Cloning*</td>
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<td>Stock</td>
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<td>Stock Storage</td>
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<td>Notes</td>
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</tr>
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<tr>
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<td>'''Ampicillin (Amp)'''</td>
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<td>50 ug/mL</td>
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<td>4°C</td>
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<td>2 months</td>
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<td>20 ug/mL stringent<br>50 ug/mL relaxed</td>
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<td>50 mg/mL (1000x) in H2O</td>
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<td>-20°C</td>
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<td>Dissolve Na salt, not free base!</td>
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</tr>
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<tr>
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<td>'''Kanamycin (Km)'''</td>
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<td>50 ug/mL</td>
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<td>4°C</td>
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<td>4-6 weeks</td>
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<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
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<td>50 mg/mL (1000x) in H2O</td>
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<td>-20°C</td>
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<td></td>
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</tr>
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<tr>
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<td>'''Chloramphenicol (Cm)''' </td>
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<td>25 ug/mL</td>
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<td>4°C</td>
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<td>at least 1 month</td>
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<td>25 ug/mL stringent<br>170 ug/mL relaxed</td>
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<td>25 mg/mL (1000x) in EtOH</td>
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<td>-20°C</td>
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<td></td>
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</tr>
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<tr>
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<td>'''Penicillin (Pen)''' </td>
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<td>1000 ug/mL</td>
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<td>4°C</td>
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<td>unknown</td>
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<td></td>
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<td>100 mg/mL (1000x) in H2O</td>
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<td>-20°C</td>
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<td>This concentration is for 1 hr lysis</td>
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</tr>
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<tr>
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<td>'''Tetracycline (Tet)'''</td>
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<td>30 ug/mL</td>
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<td>4°C</td>
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<td>unknown</td>
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<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
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<td>3 mg/mL (100x) in EtOH</td>
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<td>-20°C</td>
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<td>This concentration is for Unc knockouts<br>Very hard to dissolve</td>
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</tr>
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<tr>
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<td>'''Streptomycin (Str)'''</td>
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<td>10 ug/mL</td>
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<td>4°C</td>
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<td>unknown</td>
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<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
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<td>10 mg/mL (1000x) in H2O</td>
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<td>-20°C</td>
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<td></td>
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</tr>
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</table>
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== Notes ==
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All stocks solutions should be filter sterilized, '''not autoclaved!'''<br>
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Stocks dissolved in EtOH do not need to be filtered<br>
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All stocks should be stored at -20°<br>
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All plates and media with antibiotics should be stored at 4°<br>
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γ/δ means g/L or mg/mL<br>
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Dry antibiotic powders should be stored at 4° in a humidity-free container<br>
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*Molecular Cloning appendix A2.6 (Vol 3)<br>

Current revision

Antibiotic Typical Concentration Store At Good For Molecular Cloning* Stock Stock Storage Notes
Ampicillin (Amp) 50 ug/mL 4°C 2 months 20 ug/mL stringent
50 ug/mL relaxed
50 mg/mL (1000x) in H2O -20°C Dissolve Na salt, not free base!
Kanamycin (Km) 50 ug/mL 4°C 4-6 weeks 10 ug/mL stringent
50 ug/mL relaxed
50 mg/mL (1000x) in H2O -20°C
Chloramphenicol (Cm) 25 ug/mL 4°C at least 1 month 25 ug/mL stringent
170 ug/mL relaxed
25 mg/mL (1000x) in EtOH -20°C
Penicillin (Pen) 1000 ug/mL 4°C unknown 100 mg/mL (1000x) in H2O -20°C This concentration is for 1 hr lysis
Tetracycline (Tet) 30 ug/mL 4°C unknown 10 ug/mL stringent
50 ug/mL relaxed
3 mg/mL (100x) in EtOH -20°C This concentration is for Unc knockouts
Very hard to dissolve
Streptomycin (Str) 10 ug/mL 4°C unknown 10 ug/mL stringent
50 ug/mL relaxed
10 mg/mL (1000x) in H2O -20°C


Notes

All stocks solutions should be filter sterilized, not autoclaved!
Stocks dissolved in EtOH do not need to be filtered
All stocks should be stored at -20°
All plates and media with antibiotics should be stored at 4°
γ/δ means g/L or mg/mL
Dry antibiotic powders should be stored at 4° in a humidity-free container

  • Molecular Cloning appendix A2.6 (Vol 3)
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