Liphardt: Antibiotics: Difference between revisions
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<td>Antibiotic</td> | <td>Antibiotic</td> | ||
<td>Typical Concentration</td> | <td>Typical Concentration</td> | ||
<td>Store At</td> | |||
<td>Good For</td> | |||
<td>Molecular Cloning*</td> | |||
<td>Stock</td> | |||
<td>Stock Storage</td> | |||
<td>Notes</td> | |||
</tr> | </tr> | ||
<tr> | |||
<td>'''Ampicillin (Amp)'''</td> | |||
<td>50 ug/mL</td> | |||
<td>4°C</td> | |||
<td>2 months</td> | |||
<td>20 ug/mL stringent<br>50 ug/mL relaxed</td> | |||
<td>50 mg/mL (1000x) in H2O</td> | |||
<td>-20°C</td> | |||
<td>Dissolve Na salt, not free base!</td> | |||
</tr> | |||
<tr> | |||
<td>'''Kanamycin (Km)'''</td> | |||
<td>50 ug/mL</td> | |||
<td>4°C</td> | |||
<td>4-6 weeks</td> | |||
<td>10 ug/mL stringent<br>50 ug/mL relaxed</td> | |||
<td>50 mg/mL (1000x) in H2O</td> | |||
<td>-20°C</td> | |||
<td></td> | |||
</tr> | |||
<tr> | |||
<td>'''Chloramphenicol (Cm)''' </td> | |||
<td>25 ug/mL</td> | |||
<td>4°C</td> | |||
<td>at least 1 month</td> | |||
<td>25 ug/mL stringent<br>170 ug/mL relaxed</td> | |||
<td>25 mg/mL (1000x) in EtOH</td> | |||
<td>-20°C</td> | |||
<td></td> | |||
</tr> | |||
<tr> | |||
<td>'''Penicillin (Pen)''' </td> | |||
<td>1000 ug/mL</td> | |||
<td>4°C</td> | |||
<td>unknown</td> | |||
<td></td> | |||
<td>100 mg/mL (1000x) in H2O</td> | |||
<td>-20°C</td> | |||
<td>This concentration is for 1 hr lysis</td> | |||
</tr> | |||
<tr> | |||
<td>'''Tetracycline (Tet)'''</td> | |||
<td>30 ug/mL</td> | |||
<td>4°C</td> | |||
<td>unknown</td> | |||
<td>10 ug/mL stringent<br>50 ug/mL relaxed</td> | |||
<td>3 mg/mL (100x) in EtOH</td> | |||
<td>-20°C</td> | |||
<td>This concentration is for Unc knockouts<br>Very hard to dissolve</td> | |||
</tr> | |||
<tr> | |||
<td>'''Streptomycin (Str)'''</td> | |||
<td>10 ug/mL</td> | |||
<td>4°C</td> | |||
<td>unknown</td> | |||
<td>10 ug/mL stringent<br>50 ug/mL relaxed</td> | |||
<td>10 mg/mL (1000x) in H2O</td> | |||
<td>-20°C</td> | |||
<td></td> | |||
</tr> | |||
</table> | </table> | ||
γ/δ means g/L or mg/mL | == Notes == | ||
All stocks solutions should be filter sterilized, '''not autoclaved!'''<br> | |||
Stocks dissolved in EtOH do not need to be filtered<br> | |||
All stocks should be stored at -20°<br> | |||
All plates and media with antibiotics should be stored at 4°<br> | |||
γ/δ means g/L or mg/mL<br> | |||
Dry antibiotic powders should be stored at 4° in a humidity-free container<br> | |||
*Molecular Cloning appendix A2.6 (Vol 3)<br> | |||
Latest revision as of 18:47, 5 February 2008
| Antibiotic | Typical Concentration | Store At | Good For | Molecular Cloning* | Stock | Stock Storage | Notes |
| Ampicillin (Amp) | 50 ug/mL | 4°C | 2 months | 20 ug/mL stringent 50 ug/mL relaxed |
50 mg/mL (1000x) in H2O | -20°C | Dissolve Na salt, not free base! |
| Kanamycin (Km) | 50 ug/mL | 4°C | 4-6 weeks | 10 ug/mL stringent 50 ug/mL relaxed |
50 mg/mL (1000x) in H2O | -20°C | |
| Chloramphenicol (Cm) | 25 ug/mL | 4°C | at least 1 month | 25 ug/mL stringent 170 ug/mL relaxed |
25 mg/mL (1000x) in EtOH | -20°C | |
| Penicillin (Pen) | 1000 ug/mL | 4°C | unknown | 100 mg/mL (1000x) in H2O | -20°C | This concentration is for 1 hr lysis | |
| Tetracycline (Tet) | 30 ug/mL | 4°C | unknown | 10 ug/mL stringent 50 ug/mL relaxed |
3 mg/mL (100x) in EtOH | -20°C | This concentration is for Unc knockouts Very hard to dissolve |
| Streptomycin (Str) | 10 ug/mL | 4°C | unknown | 10 ug/mL stringent 50 ug/mL relaxed |
10 mg/mL (1000x) in H2O | -20°C |
Notes
All stocks solutions should be filter sterilized, not autoclaved!
Stocks dissolved in EtOH do not need to be filtered
All stocks should be stored at -20°
All plates and media with antibiotics should be stored at 4°
γ/δ means g/L or mg/mL
Dry antibiotic powders should be stored at 4° in a humidity-free container
- Molecular Cloning appendix A2.6 (Vol 3)
