Liphardt: Antibiotics
From OpenWetWare
(Difference between revisions)
| Line 25: | Line 25: | ||
<tr> | <tr> | ||
<td>'''Kanamycin (Km)'''</td> | <td>'''Kanamycin (Km)'''</td> | ||
| - | <td>25 ug/mL</td> | + | <td>25 or 50 ug/mL</td> |
<td>4°C</td> | <td>4°C</td> | ||
<td>4-6 weeks</td> | <td>4-6 weeks</td> | ||
<td>10 ug/mL stringent<br>50 ug/mL relaxed</td> | <td>10 ug/mL stringent<br>50 ug/mL relaxed</td> | ||
| - | <td>10 mg/mL (400x) in H2O</td> | + | <td>10 mg/mL (400x) or 25 mg/mL (1000x) in H2O</td> |
<td>-20°C</td> | <td>-20°C</td> | ||
<td></td> | <td></td> | ||
Revision as of 13:18, 10 July 2007
| Antibiotic | Typical Concentration | Store At | Good For | Molecular Cloning | Stock | Stock Storage | Notes |
| Ampicillin (Amp) | 50 ug/mL | 4°C | 2 months | 20 ug/mL stringent 50 ug/mL relaxed |
50 mg/mL (1000x) in H2O | -20°C | Dissolve Na salt, not free base! |
| Kanamycin (Km) | 25 or 50 ug/mL | 4°C | 4-6 weeks | 10 ug/mL stringent 50 ug/mL relaxed |
10 mg/mL (400x) or 25 mg/mL (1000x) in H2O | -20°C | |
| Chloramphenicol (Cm) | 25 ug/mL | 4°C | at least 1 month | 25 ug/mL stringent 170 ug/mL relaxed |
25 mg/mL (1000x) in EtOH | -20°C | |
| Penicillin (Pen) | 1000 ug/mL | 4°C | unknown | 100 mg/mL (1000x) in H2O | -20°C | This concentration is for 1 hr lysis | |
| Streptomycin (Str) | 10 ug/mL | 4°C | unknown | 10 ug/mL stringent 50 ug/mL relaxed |
10 mg/mL (1000x) in H2O | -20°C |
Notes
All stocks solutions should be filter sterilized, not autoclaved!
Stocks dissolved in EtOH do not need to be filtered
All stocks should be stored at -20°
All plates and media with antibiotics should be stored at 4°
γ/δ means g/L or mg/mL
Dry antibiotic powders should be stored at 4° in a humidity-free container
