Liphardt: Antibiotics - Revision history

Dgreenf at 01:47, 6 February 2008

Dgreenf — Wed, 06 Feb 2008 01:47:36 GMT

←Older revision		Revision as of 01:47, 6 February 2008
Line 62:		Line 62:
	<td>unknown</td>		<td>unknown</td>
	<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>		<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
-	<td>5 mg/mL (~~167x~~) in EtOH</td>	+	<td>3 mg/mL (100x) in EtOH</td>
	<td>-20°C</td>		<td>-20°C</td>
-	<td>This concentration is for Unc knockouts</td>	+	<td>This concentration is for Unc knockouts<br>Very hard to dissolve</td>
	</tr>		</tr>

Dgreenf at 01:11, 6 February 2008

Dgreenf — Wed, 06 Feb 2008 01:11:09 GMT

←Older revision		Revision as of 01:11, 6 February 2008
Line 6:		Line 6:
	<td>Store At</td>		<td>Store At</td>
	<td>Good For</td>		<td>Good For</td>
-	<td>Molecular Cloning</td>	+	<td>Molecular Cloning*</td>
	<td>Stock</td>		<td>Stock</td>
	<td>Stock Storage</td>		<td>Stock Storage</td>
Line 54:		Line 54:
	<td>-20°C</td>		<td>-20°C</td>
	<td>This concentration is for 1 hr lysis</td>		<td>This concentration is for 1 hr lysis</td>
		+	</tr>
		+
		+	<tr>
		+	<td>'''Tetracycline (Tet)'''</td>
		+	<td>30 ug/mL</td>
		+	<td>4°C</td>
		+	<td>unknown</td>
		+	<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
		+	<td>5 mg/mL (167x) in EtOH</td>
		+	<td>-20°C</td>
		+	<td>This concentration is for Unc knockouts</td>
	</tr>		</tr>

Line 80:		Line 91:
	γ/δ means g/L or mg/mL<br>		γ/δ means g/L or mg/mL<br>
	Dry antibiotic powders should be stored at 4° in a humidity-free container<br>		Dry antibiotic powders should be stored at 4° in a humidity-free container<br>
		+	*Molecular Cloning appendix A2.6 (Vol 3)<br>

Dgreenf at 19:35, 31 July 2007

Dgreenf — Tue, 31 Jul 2007 19:35:22 GMT

←Older revision		Revision as of 19:35, 31 July 2007
Line 25:		Line 25:
	<tr>		<tr>
	<td>'''Kanamycin (Km)'''</td>		<td>'''Kanamycin (Km)'''</td>
-	<td>~~25 or~~ 50 ug/mL</td>	+	<td>50 ug/mL</td>
	<td>4°C</td>		<td>4°C</td>
	<td>4-6 weeks</td>		<td>4-6 weeks</td>
	<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>		<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
-	<td>~~10 mg/mL (400x) or 25~~ mg/mL (1000x) in H2O</td>	+	<td>50 mg/mL (1000x) in H2O</td>
	<td>-20°C</td>		<td>-20°C</td>
	<td></td>		<td></td>

Dgreenf at 17:18, 10 July 2007

Dgreenf — Tue, 10 Jul 2007 17:18:15 GMT

←Older revision		Revision as of 17:18, 10 July 2007
Line 25:		Line 25:
	<tr>		<tr>
	<td>'''Kanamycin (Km)'''</td>		<td>'''Kanamycin (Km)'''</td>
-	<td>25 ug/mL</td>	+	<td>25 or 50 ug/mL</td>
	<td>4°C</td>		<td>4°C</td>
	<td>4-6 weeks</td>		<td>4-6 weeks</td>
	<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>		<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
-	<td>10 mg/mL (400x) in H2O</td>	+	<td>10 mg/mL (400x) or 25 mg/mL (1000x) in H2O</td>
	<td>-20°C</td>		<td>-20°C</td>
	<td></td>		<td></td>

Dgreenf at 00:09, 24 October 2006

Dgreenf — Tue, 24 Oct 2006 00:09:28 GMT

←Older revision		Revision as of 00:09, 24 October 2006
Line 4:		Line 4:
	<td>Antibiotic</td>		<td>Antibiotic</td>
	<td>Typical Concentration</td>		<td>Typical Concentration</td>
		+	<td>Store At</td>
		+	<td>Good For</td>
		+	<td>Molecular Cloning</td>
		+	<td>Stock</td>
		+	<td>Stock Storage</td>
		+	<td>Notes</td>
	</tr>		</tr>
-	~~</table>~~

-	'''Ampicillin'''<br>	+	<tr>
-	50 ug/mL<br>	+	<td>'''Ampicillin (Amp)'''</td>
-	~~Stock: dissolve salt~~ (not free base) 50 mg/mL in ~~millipore water, filter sterilize, store at~~ -~~20°~~<br>	+	<td>50 ug/mL</td>
-	~~Store plates~~/~~media~~ at ~~4°, good~~ for ~~2 months~~<br>	+	<td>4°C</td>
		+	<td>2 months</td>
		+	<td>20 ug/mL stringent<br>50 ug/mL relaxed</td>
		+	<td>50 mg/mL (1000x) in H2O</td>
		+	<td>-20°C</td>
		+	<td>Dissolve Na salt, not free base!</td>
		+	</tr>
		+
		+	<tr>
		+	<td>'''Kanamycin (Km)'''</td>
		+	<td>25 ug/mL</td>
		+	<td>4°C</td>
		+	<td>4-6 weeks</td>
		+	<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
		+	<td>10 mg/mL (400x) in H2O</td>
		+	<td>-20°C</td>
		+	<td></td>
		+	</tr>
		+
		+	<tr>
		+	<td>'''Chloramphenicol (Cm)''' </td>
		+	<td>25 ug/mL</td>
		+	<td>4°C</td>
		+	<td>at least 1 month</td>
		+	<td>25 ug/mL stringent<br>170 ug/mL relaxed</td>
		+	<td>25 mg/mL (1000x) in EtOH</td>
		+	<td>-20°C</td>
		+	<td></td>
		+	</tr>
		+
		+	<tr>
		+	<td>'''Penicillin (Pen)''' </td>
		+	<td>1000 ug/mL</td>
		+	<td>4°C</td>
		+	<td>unknown</td>
		+	<td></td>
		+	<td>100 mg/mL (1000x) in H2O</td>
		+	<td>-20°C</td>
		+	<td>This concentration is for 1 hr lysis</td>
		+	</tr>
		+
		+	<tr>
		+	<td>'''Streptomycin (Str)'''</td>
		+	<td>10 ug/mL</td>
		+	<td>4°C</td>
		+	<td>unknown</td>
		+	<td>10 ug/mL stringent<br>50 ug/mL relaxed</td>
		+	<td>10 mg/mL (1000x) in H2O</td>
		+	<td>-20°C</td>
		+	<td></td>
		+	</tr>
		+
		+
		+
		+	</table>

-	~~'''Kanamycin'''<br>~~
-	~~25 ug/mL<br>~~
-	~~Store plates/media at 4°, good for 1 month<br>~~


-	γ/δ means g/L or mg/mL	+	== Notes ==
		+	All stocks solutions should be filter sterilized, '''not autoclaved!'''<br>
		+	Stocks dissolved in EtOH do not need to be filtered<br>
		+	All stocks should be stored at -20°<br>
		+	All plates and media with antibiotics should be stored at 4°<br>
		+	γ/δ means g/L or mg/mL<br>
		+	Dry antibiotic powders should be stored at 4° in a humidity-free container<br>

Dgreenf at 23:46, 23 October 2006

Dgreenf — Mon, 23 Oct 2006 23:46:02 GMT

←Older revision		Revision as of 23:46, 23 October 2006
Line 1:		Line 1:
		+	<table border="1" cellpadding="5" cellspacing="0">
		+
		+	<tr>
		+	<td>Antibiotic</td>
		+	<td>Typical Concentration</td>
		+	</tr>
		+	</table>
		+
	'''Ampicillin'''<br>		'''Ampicillin'''<br>
	50 ug/mL<br>		50 ug/mL<br>
Line 7:		Line 15:
	25 ug/mL<br>		25 ug/mL<br>
	Store plates/media at 4°, good for 1 month<br>		Store plates/media at 4°, good for 1 month<br>
		+
		+
		+	γ/δ means g/L or mg/mL

Dgreenf at 21:52, 29 September 2006

Dgreenf — Fri, 29 Sep 2006 21:52:55 GMT

New page

'''Ampicillin''' 
50 ug/mL 
Stock: dissolve salt (not free base) 50 mg/mL in millipore water, filter sterilize, store at -20° 
Store plates/media at 4°, good for 2 months 

'''Kanamycin''' 
25 ug/mL 
Store plates/media at 4°, good for 1 month