Liphardt: Antibiotics

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Antibiotic Typical Concentration Store At Good For Molecular Cloning* Stock Stock Storage Notes
Ampicillin (Amp) 50 ug/mL 4°C 2 months 20 ug/mL stringent
50 ug/mL relaxed
50 mg/mL (1000x) in H2O -20°C Dissolve Na salt, not free base!
Kanamycin (Km) 50 ug/mL 4°C 4-6 weeks 10 ug/mL stringent
50 ug/mL relaxed
50 mg/mL (1000x) in H2O -20°C
Chloramphenicol (Cm) 25 ug/mL 4°C at least 1 month 25 ug/mL stringent
170 ug/mL relaxed
25 mg/mL (1000x) in EtOH -20°C
Penicillin (Pen) 1000 ug/mL 4°C unknown 100 mg/mL (1000x) in H2O -20°C This concentration is for 1 hr lysis
Tetracycline (Tet) 30 ug/mL 4°C unknown 10 ug/mL stringent
50 ug/mL relaxed
3 mg/mL (100x) in EtOH -20°C This concentration is for Unc knockouts
Very hard to dissolve
Streptomycin (Str) 10 ug/mL 4°C unknown 10 ug/mL stringent
50 ug/mL relaxed
10 mg/mL (1000x) in H2O -20°C


Notes

All stocks solutions should be filter sterilized, not autoclaved!
Stocks dissolved in EtOH do not need to be filtered
All stocks should be stored at -20°
All plates and media with antibiotics should be stored at 4°
γ/δ means g/L or mg/mL
Dry antibiotic powders should be stored at 4° in a humidity-free container

  • Molecular Cloning appendix A2.6 (Vol 3)
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