Lissa1: August6-August14

August 7

1. Pour new SUMO gel
2. Pour new small gels
3. Set up overnights for the following experiments:
   1. Nocodazole arrest
   2. Induction experiment
   3. Make more unarrested 403 for phosphatase Western
4. Make media for the nocodazole arrest.
5. Plan EVERYTHING
   1. Cloning
   2. Finalized experiments
   3. What to do about luminol/ECF deal?
6. Integrate new-found numbers/data in to model
   1. Maybe update model to include Michaelis-Menton kinetics

August 8

1. Do nocodazole arrest! -CELLS WON'T GROW:(
2. Do induction experiment! -DONE
3. Set up overnights for the following experiments: -MOVED UNTIL TOMORROW
   1. Fus3/Active Fus3 gels
   2. Phosphatase control
4. PCR up pGEV out of ACLY700 -DONE
5. Run a gel of the PCR -DONE
6. If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data) -DONE

August 9

1. PCR amplify the pGEV band I purified yesterday
2. Digest pGEV
3. Setup overnight of pRS-405 in DH5alpha, in LB + Amp
4. Prep phosphatase and Fus3/Active Fus3 samples - MOVED TO TOMORROW
5. Load samples. Start SUMO. - MOVED TO TOMORROW
6. Run Fus3/ Active Fus3 gel, and set up transfer. -MOVED TO TOMORROW

• Unfortunately, I've got a cold and need to rest:(

August 10

1. Long incubation, wash, image Fus3 gel.

August 11

1. Cut SUMO gel, set up transfer.

August 12

1. Wash and image SUMO gel.