Lissa1: August6-August14

August 7

1. Pour new SUMO gel -DONE
2. Pour new small gels -DONE
3. Set up overnights for the following experiments:
   1. Nocodazole arrest -DONE
   2. Induction experiment -DONE
   3. Make more unarrested 403 for phosphatase Western -DONE
4. Make media for the nocodazole arrest. -DONE
5. Plan EVERYTHING -CHECK
   1. Cloning
   2. Finalized experiments
   3. What to do about luminol/ECF deal? - OOPS, stil don't know
6. Integrate new-found numbers/data in to model -DONE
   1. Maybe update model to include Michaelis-Menton kinetics -NOT YET, talk to Ty

August 8

1. Do nocodazole arrest! -CELLS WON'T GROW:(
2. Do induction experiment! -DONE
3. Set up overnights for the following experiments: -MOVED UNTIL TOMORROW
   1. Fus3/Active Fus3 gels
   2. Phosphatase control
4. PCR up pGEV out of ACLY700 -DONE
5. Run a gel of the PCR -DONE
6. If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data) -DONE

August 9

1. PCR amplify the pGEV band I purified yesterday
2. Digest pGEV
3. Setup overnight of pRS-405 in DH5alpha, in LB + Amp
4. Prep phosphatase and Fus3/Active Fus3 samples - MOVED TO TOMORROW
5. Load samples. Start SUMO. - MOVED TO TOMORROW
6. Run Fus3/ Active Fus3 gel, and set up transfer. -MOVED TO TOMORROW

• Unfortunately, I've got a cold and need to rest:(

August 10

1. Long incubation, wash, image Fus3 gel.

August 11

1. Cut SUMO gel, set up transfer.

August 12

1. Wash and image SUMO gel.