Lissa1: August6-August14: Difference between revisions
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==August 9== | ==August 9== | ||
#PCR amplify the pGEV band I purified yesterday | #PCR amplify the pGEV band I purified yesterday -DONE | ||
#PCR out GEV insert from the original pGEV DNA -DONE | |||
#Run gels of these PCRs and do gel extraction | |||
#Digest pGEV | #Digest pGEV | ||
#Setup overnight of pRS-405 in DH5alpha, in LB + Amp | #Setup overnight of pRS-405 in DH5alpha, in LB + Amp | ||
# | #Setup overnight of samples for Fus3, etc -DONE | ||
*Unfortunately, I've got a cold and need to rest:( | *Unfortunately, I've got a cold and need to rest:( |
Revision as of 13:00, 9 August 2006
August 6
August 7
- Pour new SUMO gel -DONE
- Pour new small gels -DONE
- Set up overnights for the following experiments:
- Nocodazole arrest -DONE
- Induction experiment -DONE
- Make more unarrested 403 for phosphatase Western -DONE
- Make media for the nocodazole arrest. -DONE
- Plan EVERYTHING -CHECK
- Cloning
- Finalized experiments
- What to do about luminol/ECF deal? - OOPS, stil don't know
- Integrate new-found numbers/data in to model -DONE
- Maybe update model to include Michaelis-Menton kinetics -NOT YET, talk to Ty
August 8
- Do nocodazole arrest! -CELLS WON'T GROW:(
- Do induction experiment! -DONE
- Set up overnights for the following experiments: -MOVED UNTIL TOMORROW
- Fus3/Active Fus3 gels
- Phosphatase control
- PCR up pGEV out of ACLY700 -DONE
- Run a gel of the PCR -DONE
- If there's time, run a second gel and extract and purify the pGEV (which might not be pGEV, based on the sequencing data) -DONE
August 9
- PCR amplify the pGEV band I purified yesterday -DONE
- PCR out GEV insert from the original pGEV DNA -DONE
- Run gels of these PCRs and do gel extraction
- Digest pGEV
- Setup overnight of pRS-405 in DH5alpha, in LB + Amp
- Setup overnight of samples for Fus3, etc -DONE
- Unfortunately, I've got a cold and need to rest:(
August 10
- Long incubation, wash, image Fus3 gel.
August 11
- Cut SUMO gel, set up transfer.
August 12
- Wash and image SUMO gel.